Screening of potato breedung lines for the potato virus Y resistance genes Rychc and Ryadg

Goal. Molecular identification of carriers the potato virus Y (PVY) resistance genes Rychc and Ryadg among lines Polissia Research Station Institute Potato Growing National Academy Agrarian Sciences Ukraine.
 Methods. Seventy produced by were studied. We used molecular markers indicating presence specific Ry in genome lines. Those are responsible for plant’s response to pathogen extreme (ER) type: Ryo186 marker gene, RYSC-3 determination gene. The gene chc, derives from Solanum chacoense, S. tuberosum ssp. andigena. For determining PVY genes, PCR analysis was used, products analyzed electrophoresis an agarose gel supplemented with ethidium bromide visualize amplicons.
 Results. Screening alleles Ruo186 showed that 53 (75.72%) carried Rychc. allele identified 7 (10%) using marker. Among sample, there three (4.2%) which simultaneously both genes: P.17.36-8, P.16.21-8, P.17.21/36. When compared studies other authors, our sample breeding we have mostly a similar percentage occurrence much higher frequency gene.
 Conclusions. Thus, potential most strains, including PVYNTN strain.